Affinity-repulsion chromatography. Principle and application to lectins.
نویسندگان
چکیده
منابع مشابه
Affinity-repulsion chromatography. Principle and application to lectins.
The interactions of proteins with their immobilized ligands in an electrically charged microenvironment were studied. The binding of lectins to erythrocytes and to affinity matrices was used as a model system. Lectins bind and agglutinate erythrocytes in the presence of at least 10 mM NaCl or 1 mM CaCl2, but not in deionized water. The salt dependence of the agglutination process is due to the ...
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Effects of Lectins Affinity Chromatography on Glycoproteins Enrichment Using AP-MALDI .ppt
EXPERIMENTAL Glycosylation is a common coand posttranslational modification of proteins, which may have profound effects on protein structure and function [1-3]. In glycoproteins, different carbohydrates can be attached to different position on the protein back bone at a given time. In addition, different substituents such as acetyl, lactyl, formyl and sulfate can modify a single carbohydrate t...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1988
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)68188-8